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Registro Completo |
Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
Data corrente: |
28/07/2010 |
Data da última atualização: |
18/08/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LOPES, M. A.; HORA JUNIOR, B. T.; DIAS, C. V.; SANTOS, G. C.; GRAMACHO, K. P.; CASCARDO, J. C. M.; GESTEIRA, A. da S.; MICHELI, F. |
Afiliação: |
Karina Peres Gramacho, CEPLAC; Júlio Cézar Mattos Cascardo, UESC; ABELMON DA SILVA GESTEIRA, CNPMF; Fabienne Micheli, UESC. |
Título: |
Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae). |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Genetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010. |
ISSN: |
1676-5680 |
DOI: |
10.4238/vol9-3gmr825 |
Idioma: |
Inglês |
Conteúdo: |
Cacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees. |
Palavras-Chave: |
Gene regulation; Macroarrays; RT-qPCR; Theobrama cacao; Witches broom disease. |
Thesagro: |
Cacau; Doença de Planta; Fungo; Genótipo; Hibridação; Vassoura de Bruxa. |
Categoria do assunto: |
G Melhoramento Genético |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/858659/1/gmr825.pdf
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Marc: |
LEADER 02501naa a2200361 a 4500 001 1858659 005 2022-08-18 008 2010 bl uuuu u00u1 u #d 022 $a1676-5680 024 7 $a10.4238/vol9-3gmr825$2DOI 100 1 $aLOPES, M. A. 245 $aExpression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae).$h[electronic resource] 260 $c2010 520 $aCacao (Theobroma cacao) is one of the most important tropical crops; however, production is threatened by numerous pathogens, including the hemibiotrophic fungus Moniliophthora perniciosa, which causes witches? broom disease. To understand the mechanisms that lead to the development of this disease in cacao, we focused our attention on cacao transcription factors (TFs), which act as master regulators of cellular processes and are important for the fine-tuning of plant defense responses. We developed a macroarray with 88 TF cDNA from previously obtained cacao-M. perniciosa interaction libraries. Seventy-two TFs were found differentially expressed between the susceptible (Catongo) and resistant (TSH1188) genotypes and/or during the disease time course - from 24 h to 30 days after infection. Most of the differentially expressed TFs belonged to the bZIP, MYB and WRKY families and presented opposite expression patterns in susceptible and resistant cacao-M. perniciosa interactions (i.e., up-regulated in Catongo and down-regulated in TSH1188). The results of the macroarray were confirmed for bZIP and WRKY TFs by real-time PCR. These differentially expressed TFs are good candidates for subsequent functional analysis as well as for plant engineering. Some of these TFs could also be localized on the cacao reference map related to witches? broom resistance, facilitating the breeding and selection of resistant cacao trees. 650 $aCacau 650 $aDoença de Planta 650 $aFungo 650 $aGenótipo 650 $aHibridação 650 $aVassoura de Bruxa 653 $aGene regulation 653 $aMacroarrays 653 $aRT-qPCR 653 $aTheobrama cacao 653 $aWitches broom disease 700 1 $aHORA JUNIOR, B. T. 700 1 $aDIAS, C. V. 700 1 $aSANTOS, G. C. 700 1 $aGRAMACHO, K. P. 700 1 $aCASCARDO, J. C. M. 700 1 $aGESTEIRA, A. da S. 700 1 $aMICHELI, F. 773 $tGenetics and Molecualr Reseach, Ribeirão Preto$gv. 9, n. 3, p. 1279-1297, 2010.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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1. | | CASA-COILA, V. H.; LEHNER, M. S.; HORA JUNIOR, B. T.; REIS, A.; NAZARENO, N. R. X.; MIZUBUTI, E. S. G.; GOMES, C. B. First report of Phytophthora infestans self fertile genotypes in southern Brazil Plant Disease, v. 101, n. 9, p. 1682, Sept. 2017.Tipo: Nota Técnica/Nota Científica | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Clima Temperado; Embrapa Hortaliças. |
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2. | | LOPES, M. A.; HORA JUNIOR, B. T.; DIAS, C. V.; SANTOS, G. C.; GRAMACHO, K. P.; CASCARDO, J. C. M.; GESTEIRA, A. da S.; MICHELI, F. Expression analysis of transcription factors from the interaction between cacao and Moniliophthora perniciosa (Tricholomataceae). Genetics and Molecualr Reseach, Ribeirão Preto, v. 9, n. 3, p. 1279-1297, 2010.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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3. | | HORA JUNIOR, B. T. da; POLONI, J. de F.; LOPES, M. A.; DIAS, C. V.; GRAMACHO, K. P.; SCHUSTER, I.; SABAU, X.; CASCARDO, J. C. de M.; DI MAURO, S. M. Z.; GESTEIRA, A. da S.; BONATTO, D.; MICHELI, F. Transcriptomics and systems biology analysis in identification of specific pathways involved in cacao resistance and susceptibility to witches. Molecular BioSystems, n.8, p. 1507-1519, 2012Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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4. | | SOARES, V. L. F.; RODRIGUES, S. M.; OLIVEIRA, T. M. de; QUEIROZ, T. O. de; LIMA, L. S.; HORA-JÚNIOR, B. T.; GRAMACHO, K. P.; MICHELI, F.; CASCARDO, J. C. M.; OTONI, W. C.; GESTEIRA, A. S.; COSTA, M. G. C. Unraveling new genes associated with seed development and metabolism in Bixa orellana L. by expressed sequence tag (EST) analysis. Molecular Biology Reports, New York, jun. 2010. On line first, with supplementary materialsTipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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